Journal: F1000Research

Article Title: Andrographolide, isolated from Andrographis paniculata , induces apoptosis in monocytic leukemia and multiple myeloma cells via augmentation of reactive oxygen species production

doi: 10.12688/f1000research.53595.2

Figure Lengend Snippet: The y-axis values of the cell viability histograms represent the optical density (550 nm) in comparison with the control (set as 100%) as measured by MTT assay. The optical density markedly decreased after treatment with Andro (10, 30, 50 μM), Ara-C (40 μM), or VCR (0.1 μM) compared with untreated cells in THP-1 (a) and H929 (b) cells. The results are expressed as mean ± SD of three independent experiments.

Article Snippet: THP-1 cells (human monocytic leukemia cell line; EC88081201; RRID:CVCL_0006) and NCI-H929 cells (human IgA-kappa-producing multiple myeloma cell line; EC95050415; RRID:CVCL_1600) were obtained from DS PHARMA BIOMEDICAL (Osaka, Japan).

Techniques: MTT Assay

Journal: F1000Research

Article Title: Andrographolide, isolated from Andrographis paniculata , induces apoptosis in monocytic leukemia and multiple myeloma cells via augmentation of reactive oxygen species production

doi: 10.12688/f1000research.53595.2

Figure Lengend Snippet: Morphologies of THP-1 (a) and H929 (b) cells after 24 h of treatment with Andro, Ara-C, or VCR were compared with untreated cells after Wright-Giemsa staining. White arrows indicate cells showing nuclear condensation and black scale bars represent 20 μm. (c) Nuclear DNA fragmentation was revealed by agarose gel electrophoresis of DNA isolated after 24 h of treatment with Andro (50μM), Ara-C (40μM), or VCR (0.1μM) in THP-1 (lanes 2-5) and H929 cells (lanes 6-9). Lane 1, DNA size marker; lanes 2 and 6, untreated cells; lanes 3 and 7, cells treated with Andro; lanes 4 and 8, cells treated with Ara-C; lanes 5 and 9, cells treated with VCR.

Article Snippet: THP-1 cells (human monocytic leukemia cell line; EC88081201; RRID:CVCL_0006) and NCI-H929 cells (human IgA-kappa-producing multiple myeloma cell line; EC95050415; RRID:CVCL_1600) were obtained from DS PHARMA BIOMEDICAL (Osaka, Japan).

Techniques: Staining, Agarose Gel Electrophoresis, Isolation, Marker

Journal: F1000Research

Article Title: Andrographolide, isolated from Andrographis paniculata , induces apoptosis in monocytic leukemia and multiple myeloma cells via augmentation of reactive oxygen species production

doi: 10.12688/f1000research.53595.2

Figure Lengend Snippet: Cell cycle phases of individual cells were measured after treatment for 24 h with Andro (50μM), Ara-C (40μM), or VCR (0.1μM) using the Muse Cell Analyzer. In contrast to Ara-C and VCR, treatment with Andro hardly affected the percentages of THP-1 or H929 cells found in the G0/G1, S, and G2/M phases. Percentages are expressed as mean of three independent experiments. For statistical analysis the percentages of cells in the G0/G1 phase were compared (*P < 0.05, **P < 0.01, ***P < 0.001).

Article Snippet: THP-1 cells (human monocytic leukemia cell line; EC88081201; RRID:CVCL_0006) and NCI-H929 cells (human IgA-kappa-producing multiple myeloma cell line; EC95050415; RRID:CVCL_1600) were obtained from DS PHARMA BIOMEDICAL (Osaka, Japan).

Techniques:

Journal: F1000Research

Article Title: Andrographolide, isolated from Andrographis paniculata , induces apoptosis in monocytic leukemia and multiple myeloma cells via augmentation of reactive oxygen species production

doi: 10.12688/f1000research.53595.2

Figure Lengend Snippet: Cell viability was measured after 24 h treatment with Andro, Ara-C, or VCR of THP-1 (a) and H929 (b) cells in the presence or absence of the ROS production inhibitor NAC. The y-axis values represent the optical density (550 nm) in comparison with the control (set as 100%) as measured by MTT assay.

Article Snippet: THP-1 cells (human monocytic leukemia cell line; EC88081201; RRID:CVCL_0006) and NCI-H929 cells (human IgA-kappa-producing multiple myeloma cell line; EC95050415; RRID:CVCL_1600) were obtained from DS PHARMA BIOMEDICAL (Osaka, Japan).

Techniques: MTT Assay

Journal: Nature Communications

Article Title: Oncogenic RAS commandeers amino acid sensing machinery to aberrantly activate mTORC1 in multiple myeloma

doi: 10.1038/s41467-022-33142-x

Figure Lengend Snippet:

Article Snippet: KSM12PE , Human multiple myeloma cell line , JCRB , JCRB0430.

Techniques: Transformation Assay

Journal: Biomedicines

Article Title: Inhibiting UNC13B Suppresses Cell Proliferation by Upregulating the Apoptotic Pathway in Multiple Myeloma

doi: 10.3390/biomedicines13092086

Figure Lengend Snippet: Relative mRNA expression of UNC13B in multiple myeloma cell lines. ( A ) Quantitative real-time PCR (qPCR) analysis of UNC13B mRNA levels in U266, ARD, and RPMI 8226 cell lines. U266 was used as the reference control for relative quantification, and GAPDH served as the internal reference gene. Expression levels were calculated using the 2 -ΔΔCt method and are presented as fold-change relative to U266. The mean ± standard deviation of UNC13B expression was 1.00 ± 0.26 in U266, 3.07 ± 0.44 in ARD, and 1.41 ± 0.26 in RPMI 8226 cells. Overlaid symbols (●, ■, ▲) denote individual independent experiments ( n = 5 per group); different marker shapes are used only for visual distinction and carry no additional meaning. ( B ) Relative UNC13B mRNA expression in ARD cells following different treatments. The Scramble + 25 μM treatment group was used as the reference for comparison. **** indicate p < 0.0001; ns = not significant ( p ≥ 0.05). Data are presented as the mean ± standard deviation (SD, n = 5 independent experiments).

Article Snippet: Human multiple myeloma cell lines ARD (CL-0778), U266 (CL-0510), and RPMI 8226 (CL-0564) were obtained from Procell Life Science & Technology Co., Ltd. (Wuhan, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Control, Quantitative Proteomics, Gene Expression, Standard Deviation, Marker, Comparison